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Involvement of TLR2 in innate response to Bacillus anthracis infection

Department of Infectious Diseases, Israel Institute for Biological Research, Ness-Ziona, Israel, shayw{at}iibr.gov.il

Haim Levy

Department of Infectious Diseases, Israel Institute for Biological Research, Ness-Ziona, Israel

Morly Fisher

Department of Infectious Diseases, Israel Institute for Biological Research, Ness-Ziona, Israel

David Kobiler

Department of Infectious Diseases, Israel Institute for Biological Research, Ness-Ziona, Israel

Zeev Altboum

Department of Infectious Diseases, Israel Institute for Biological Research, Ness-Ziona, Israel

The involvement of TLR2 receptor in the innate response to infection with Bacillus anthracis was investigated. We studied the response to virulent or attenuated Vollum strains in either in vitro assays using macrophage cultures, or in an in vivo model comparing the sensitivity of Syrian hamster cells (expressing normal TLR2) to Chinese hamster cells (lacking functional TLR2) to infection by the various B. anthracis strains. Phagocytosis experiments with murine cell cultures or primary macrophages from both hamster strains, using virulent or attenuated Tox⁺Cap ^-, Tox^-Cap⁺ or Tox^-Cap^- spores indicated that the secretion of TNF- was induced by all the bacterial spores and purified spore antigens. In contrast, capsular antigens induce secretion of TNF- only by Syrian hamster macrophages indicating the involvement of a functional TLR2 in macrophage activation. Challenge experiments with both hamster strains by intranasal spore inoculation, indicated that, while both strains are equally sensitive to infection with the virulent strain, the Chinese hamster demonstrated a higher sensitivity to infection with the toxinogenic or encapsulated strains. In conclusion, our findings imply that TLR2 has an important role in the attempt of the innate immunity to control B. anthracis infection, although TNF- secretion was found to be mediated by both TLR2-dependent and TLR2-independent pathways.

Key Words: TLR2 • Bacillus anthracis • TNF- • innate immunity

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Innate Immunity, Vol. 15, No. 1, 43-51 (2009)
DOI: 10.1177/1753425908100379


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This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Weiss, S. Articles by Altboum, Z. Search for Related Content PubMed PubMed Citation Articles by Weiss, S. Articles by Altboum, Z. Social Bookmarking                         What's this?