This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Wei Cui Articles by Morrison, D. C. Search for Related Content PubMed PubMed Citation Articles by Wei Cui, Articles by Morrison, D. C. Social Bookmarking                         What's this?

Differential modulation of the induction of inflammatory mediators by antibiotics in mouse macrophages in response to viable Gram-positive and Gram-negative bacteria

Stowers Institute for Medical Research, 10000 East 50 Street, Kansas City, MO 64110, USA

Mei-Guey Lei

Department of Basic Medical Science University of Missouri-Kansas City School of Medicine

Richard Silverstein

Department of Molecular Biology and Biochemistry, Kansas University Medical Center, Kansas City, Kansas, USA

David C. Morrison

Department of Basic Medical Science University of Missouri-Kansas City School of Medicine, morrisondc{at}umkc.edu, Department of Surgery, University of Missouri-Kansas City School of Medicine, ShocK/Trauma Research Center, University of Missouri-Kansas City School of Medicine, Kansas City, Missouri, USA

We have investigated effects of ß-lactam antibiotics on TNF-, and iNOS production from mouse peritoneal macrophages following co-culture with Escherichia coli or Staphylococcus aureus bacteria. Ceftazidime and aztreonam enhanced TNF- secretion from macrophages stimulated with E. coli; however, imipenem does not alter either the kinetics or magnitude of TNF- in E. coli -treated macrophages. Similar treatments with S. aureus co-cultured with macrophages markedly altered profiles of TNF- response characterized by apparent early TNF- peak relative to untreated S. aureus. All antibiotics increased E. coli-induced iNOS expression as assessed by both mRNA and protein. These same antibiotics significantly reduced S. aureus-induced iNOS levels of RNA. Both ceftazidime and aztreonam enhanced LPS release from E. coli in comparison to low-level LPS release from imipenem-treated bacteria, consistent with observed differences in TNF- release. Incubation of all three antibiotics with S. aureus similarly increased levels of the cell wall constituent protein A detected in supernatants at early time points indicating microbial lysis. In parallel, S. aureus culture supernatants from 2-h incubation with antibiotics enhanced TNF- release. These results indicate that different cellular mechanisms contribute to antibiotic-mediated regulation of TNF- and iNOS secretion in mouse macrophages in response to E. coli versus S. aureus.

Journal of Endotoxin Research, Vol. 9, No. 4, 225-236 (2003)
DOI: 10.1177/09680519030090040301


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?