This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Miyake, K. Articles by Kosugi, A. Search for Related Content PubMed PubMed Citation Articles by Miyake, K. Articles by Kosugi, A. Social Bookmarking                         What's this?

Essential role of MD-2 in B-cell responses to lipopolysaccharide and Toll-like receptor 4 distribution

Division of Infectious Genetics, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan, kmiyake{at}ims.u-tokyo.ac.jp, CREST, Japan Science and Technology Corporation, Tokyo, Japan

Yoshinori Nagai

Division of Infectious Genetics, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan, CREST, Japan Science and Technology Corporation, Tokyo, Japan

Sachiko Akashi

Division of Infectious Genetics, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan, CREST, Japan Science and Technology Corporation, Tokyo, Japan

Masakazu Nagafuku

CREST, Japan Science and Technology Corporation, Tokyo, Japan, School of Allied Health Sciences, Faculty of Medicine, Osaka University Medical School, Osaka, Japan

Masato Ogata

Department of Oncogenesis, Osaka University Medical School, Osaka, Japan

Atsushi Kosugi

CREST, Japan Science and Technology Corporation, Tokyo, Japan, School of Allied Health Sciences, Faculty of Medicine, Osaka University Medical School, Osaka, Japan

Toll-like receptor 4 (TLR4) mediates lipopolysaccharide (LPS) signaling in a variety of cell types. MD-2 is associated with the extracellular domain of TLR4 and augments TLR4-dependent LPS responses in vitro. Moreover, mice lacking MD-2 (MD-2^{— /—}) do not respond to LPS, survive endotoxin shock, and are susceptible to Salmonella typhimurium infection. Here, we further show that B cells lacking MD-2 do not up-regulate CD23 in response to LPS. TLR4 predominantly resides in the Golgi apparatus without MD-2. MD-2 is essential for LPS responses in vivo.

Journal of Endotoxin Research, Vol. 8, No. 6, 449-452 (2002)
DOI: 10.1177/09680519020080061401


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?