Journal of Endotoxin Research

 

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Journal of Endotoxin Research, Vol. 8, No. 5, 357-364 (2002)
DOI: 10.1177/09680519020080050901

Monoclonal antibodies against 3-deoxy-{alpha}-D-manno-oct-2-ulosonic acid (Kdo) and D-glycero-{alpha}-D-talo-oct-2-ulosonic acid (Ko)

Lore Brade

Research Center Borstel, Center for Medicine and Biosciences, Borstel, Germany, hbrade{at}fz-borstel.de

Sabine Gronow

Research Center Borstel, Center for Medicine and Biosciences, Borstel, Germany

Norbert Wimmer

Institute of Chemistry, University of Agriculture, Vienna, Austria

Paul Kosma

Institute of Chemistry, University of Agriculture, Vienna, Austria

Helmut Brade

Research Center Borstel, Center for Medicine and Biosciences, Borstel, Germany

Monoclonal antibodies (mAbs) were obtained after immunization of mice with neoglycoconjugates containing as a ligand the disaccharide Kdo(2-> 4)Ko or Ko(2->4)Kdo, representing structural elements of the core region of lipopolysaccharides (LPSs) from Acinetobacter haemolyticus and Burkholderia cepacia, respectively. One antibody, S67-9, bound with high specificity to Ko(2-> 4)Kdo-BSA showing no cross reactivity with Kdo(2-> 4)Kdo-BSA and the other antigens tested. A second mAb, S68-12, bound preferentially to Kdo(2-> 4)Ko-BSA but cross reacted with Ko(2-> 4)Kdo-BSA and Kdo(2-> 4)Kdo-BSA. A third mAb, S67-27, was found to bind Kdo monosaccharide. Although mAbs S67-9 and S68-12 did not bind to LPS of Burkholderia or Acinetobacter as expected, the mAbs will be useful tools in studying the biosynthesis of LPS containing Ko.


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