This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Vodovotz, Y. Articles by Billiar, T. R. Search for Related Content PubMed PubMed Citation Articles by Vodovotz, Y. Articles by Billiar, T. R. Social Bookmarking                         What's this?

The hepatocyte as a microbial product-responsive cell

Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA

Shubing Liu

Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA

Carol McCloskey

Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA

Richard Shapiro

Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA

Angela Green

Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA

Timothy R. Billiar

Department of Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, USA, billiartr{at}msx.upmc.edu

Much research has focused on the responses to microbial products of immune cells such as monocytes, macrophages, and neutrophils. Although the liver is a primary response organ in various infections, relatively little is known about the antimicrobial responses of its major cell type, the hepatocyte. It is now known that the recognition of bacteria occurs via cell-surface proteins that are members of the Toll-like receptor (TLR) family. In addition, lipopolysaccharide (LPS) is bound by circulating LPS-binding protein (LBP) and presented to cell-surface CD14, which in turn interacts with TLR and transduces an intracellular signal. We investigated the CD14 and TLR2 responses of whole liver and isolated hepatocytes, and demonstrated that these cells can be induced to express the molecules necessary for responses to both Gram-positive and Gram-negative bacteria. Our findings may have clinical implications for pathological states such as sepsis.

Journal of Endotoxin Research, Vol. 7, No. 5, 365-373 (2001)
DOI: 10.1177/09680519010070050401


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?