Journal of Endotoxin Research

 

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Journal of Endotoxin Research, Vol. 7, No. 3, 227-231 (2001)
DOI: 10.1177/09680519010070030601

Decay-accelerating factor (DAF/CD55) is a functional active element of the LPS receptor complex

H. Heine

Center for Medicine and Biosciences, Research Center Borstel, Borstel, Germany

A.J. Ulmer

Center for Medicine and Biosciences, Research Center Borstel, Borstel, Germany, ajulmer{at}fz-borstel.de

V.T. El-Samalouti

Center for Medicine and Biosciences, Research Center Borstel, Borstel, Germany

A. Lentschat

Center for Medicine and Biosciences, Research Center Borstel, Borstel, Germany

L. Hamann

Center for Medicine and Biosciences, Research Center Borstel, Borstel, Germany

Previously, we identified an 80 kDa membrane protein (LMP80) that is capable of binding to LPS and lipid A in the presence of LBP and sCD14. LMP80 could also be detected after immuno-coprecipitation of cell membranes with LPS and lipid A, indicating a physical contact of LMP80 and LPS/lipid A. Further analysis and peptide sequencing revealed that LMP80 is identical to CD55 (decay accelerating factor, DAF), a regulatory molecule of the complement cascade. Transfection of LPS-hyporesponsive Chinese hamster ovary (CHO) cells with human CD55 resulted in the translocation of NF-{kappa}B upon stimulation with LPS or lipid A. Our results demonstrate a new functional role of CD55 as a molecule able to mediate LPS-induced activation of cells that may be part of a multimeric LPS receptor complex.


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