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Journal of Endotoxin Research
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Structural and serological characterisation of the O-antigenic polysaccharide of the lipopolysaccharide from Acinetobacter strain 96 (DNA group 11)

Evgeny V. Vinogradov

Institute for Biological Sciences, NRCC, Ottawa, Ontario, Canada

Ralph Pantophlet

The Scripps Research Institute, Department of Immunology, La Jolla, California, USA

Helmut Brade

Divisions of Medical and Biochemical Microbiology, Research Center Borstel, Borstel, Germany

Otto Holst

Analytical Biochemistry, Research Center Borstel, Borstel, Germany, oholst{at}fzborstel.de

A polysaccharide containing D-Manp, L-Fucp (6-deoxygalactopyranose, fucose) and D-Glc pNAc was isolated by mild acid hydrolysis, followed by gel-permeation chromatography, from the lipopolysaccharide derived from Acinetobacter strain 96 (DNA group 11). The structure of the O-antigen was determined by compositional analysis and NMR spectroscopy of the polysaccharide as:

A monoclonal antibody obtained after immunization of mice with heat-killed bacteria of Acinetobacter strain 96 was shown to bind to the O-antigen and did not cross-react with any Acinetobacter O-antigen of known structure.

Journal of Endotoxin Research, Vol. 7, No. 2, 113-118 (2001)
DOI: 10.1177/09680519010070020501
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