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Journal of Endotoxin Research
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Comparative study of peritoneal macrophage functions in mice receiving lethal and non-lethal doses of LPS

V.M. Víctor

Department of Animal Physiology, Faculty of Biological Sciences, Complutense University, Madrid, Spain

M. De la Fuente

Department of Animal Physiology, Faculty of Biological Sciences, Complutense University, Madrid, Spain, mondelaf{at}eucmax.sim.ucm.es

In previous studies, we have observed changes in several functions of peritoneal macrophages from female BALB/c mice with lethal endotoxic shock caused by intraperitoneal injection of Escherichia coli O55:B5 lipopolysaccharide (LPS; 100 mg/kg), which were associated with a high production of superoxide anion and tumor necrosis factor alpha (TNF-{alpha}). In the present work, both a lethal dose (250 mg/kg) and a non-lethal dose (100 mg/kg) of LPS were used in female Swiss mice. In peritoneal macrophages, the following functions were studied at 2, 4, 12 and 24 h after LPS injection: adherence to substrate, chemotaxis, ingestion of particles, and superoxide anion and TNF-{alpha} production. In both groups, the results showed a stimulation of adherence, ingestion and superoxide production as well as a decrease of chemotaxis, whereas TNF-{alpha} could not be detected in either of the two groups. These effects were more evident with the 250 mg/kg dose, especially as regards superoxide anion production, which was higher in the animals treated with a lethal dose of LPS.

Journal of Endotoxin Research, Vol. 6, No. 3, 235-241 (2000)
DOI: 10.1177/09680519000060030401
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