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Journal of Endotoxin Research
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Endotoxin-induced alterations of lipid and fatty acid compositions in rat liver peroxisomes

Mushfiquddin Khan

Department of Pediatrics, Medical University of South Carolina, Charleston, South Carolina, USA

Miguel Contreras

Department of Pediatrics, Medical University of South Carolina, Charleston, South Carolina, USA, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA

Inderjit Singh

Department of Pediatrics, Medical University of South Carolina, Charleston, South Carolina, USA, singhi{at}musc.edu

The structure/function of peroxisomal lipids in rat liver treated with a sublethal dose of endotoxin, a lipopolysaccharide (LPS), was investigated. Peroxisomes isolated from LPS-treated rat liver had remarkable alterations in lipid content compared with saline treated control liver peroxisomes. Cholesterol and phospholipids (PL) decreased significantly by 28.7% and 50.8%, respectively, leading to the change in the ratio of cholesterol/phospholipids (control 0.081 versus LPS 0.118, P <0.001). A quantitative analysis from LPS-treated rat liver peroxisomes showed a general decrease in all classes of PL. No such alterations were observed in lipid content of other subcellular organelles. The peroxisomal fatty acid composition in LPS-treated animals was also altered. An analysis of fatty acid composition in PL and phosphatidylcholine from LPS-treated peroxisomes showed an increase in arachidonic acid (C20:4) and docosahexaenoic acid (C22:6). Very long chain (VLC) fatty acids (>C22:0) were also found increased in all classes of lipids in LPS-treated peroxisomes. Gadolinium chloride (GAD) mediated inactivation of Kupffer cells (KC) normalized cholesterol/PL ratio in LPS-treated peroxisomes. Collectively, the results indicate that the peroxisome metabolism of lipids and fatty acids is specifically altered in endotoxin-treated rat liver and at least part of the alterations may be mediated by factors released by KC.

Journal of Endotoxin Research, Vol. 6, No. 1, 41-50 (2000)
DOI: 10.1177/09680519000060010601
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