This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yoshida, K. Articles by Sawada, H. Search for Related Content PubMed Articles by Yoshida, K. Articles by Sawada, H. Social Bookmarking                   What's this?

Lipopolysaccharide-induced vacuoles in macrophages: Their origin is plasma membrane-derived organelles and endoplasmic reticulum, but not lysosomes

Department of Anatomy, Yokohama City University School of Medicine, Kanazawa-ku, Yokohama, Japan

Michio Ono

Department of Anatomy, Yokohama City University School of Medicine, Kanazawa-ku, Yokohama, Japan

Hajime Sawada

Department of Anatomy, Yokohama City University School of Medicine, Kanazawa-ku, Yokohama, Japan

Lipopolysaccharide (LPS) is one of the potent activators of macrophages. LPS was shown to induce cell spreading and large vacuoles in the cytoplasm of a macrophage-like cell line, JY3. These vacuoles were negative for acid phosphatase histochemistry and did not take up Lucifer yellow added to the medium. Latex beads were incorporated into cytoplasmic vesicles distinct from the vacuoles. These results indicated that the vacuoles are neither phagosomes nor lysosomes.

DiIC₁₈(3), a specific marker of endoplasmic reticulum (ER), stained the vacuoles intensely, and DiOC₆(3) stained the vacuoles at a density similar to nuclear envelope, suggesting ER origin of their membrane. Glucose-6-phosphatase, however, was not detected histochemically.

Vacuoles were also stained with wheat germ agglutinin-horseradish peroxidase (WGA-HRP) or WGA-biotin, suggesting that the vacuoles originated from plasma membrane-endosome-trans Golgi network-secretory granule pathway. Golgi markers, TPPase or BODIPY-ceramide were not localized to the vacuolar membrane.

These results indicate that the vacuoles may have dual origins; ER and plasma membrane-derived organelles.

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				G. D. Kutuzova, R. M. Albrecht, C. M. Erickson, and N. Qureshi Diphosphoryl Lipid A from Rhodobacter sphaeroides Blocks the Binding and Internalization of Lipopolysaccharide in RAW 264.7 Cells J. Immunol., July 1, 2001; 167(1): 482 - 489. [Abstract] [Full Text] [PDF]