This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Lam, J.S. Articles by Burrows, L.L. Search for Related Content Social Bookmarking                   What's this?

Glycosyltransferases of Pseudomonas aeruginosa that assemble the O antigens of A band and B band lipopolysaccharide

Canadian Bacterial Diseases Network, Department of Microbiology, University of Guelph, Guelph, Ontario, Canada, jlam{at}uoguelph.ca

H.L. Rocchetta

Canadian Bacterial Diseases Network, Department of Microbiology, University of Guelph, Guelph, Ontario, Canada

L.L. Burrows

Canadian Bacterial Diseases Network, Department of Microbiology, University of Guelph, Guelph, Ontario, Canada

Pseudomonas aeruginosa produces two forms of lipopolysaccharide (LPS) designated A band and B band. The O-polysaccharide region of A band is a conserved D-rhamnan polymer arranged 1—2, 1—3, 1—3, while B band is serotype-specific with differences in the O-antigenic region dividing P. aeruginosa into 20 stereotypes. The B band O-antigen unit of serotype O5 is [—4)-ß-D-Man(2NAc3N)A-(1-4)-ß-D-Man(2NAc3NAc)A-(1-3)--D-Fuc2NAc]. The glycosidic structure of LPS molecules specified by the action of dedicated glycosyltransferases. The wbp clusters of A band and B band (serotype O5) were each found to contain three genes coding for putative glycosyltransferases: wbpX, wbpY, wbpZ, and wbpH, wbpJ, wbpL , respectively. To examine the role of these potential transferases in LPS assembly, chromosomal mutations were generated within all 6 genes. LPS analysis reveals that wbpX, wbpY, and wbpZ mutants express an A^—B⁺ phenotype, while wbpH and wbpJ mutants are A⁺B^—. Interestingly, mutations in wbpL, an Escherichia coli wec A homologue, abrogates both A band and B band LPS synthesis. Based on amino acid homologies, O-polysaccharide structures and LPS phenotypes of transferase mutants, we propose an assembly scheme for these two LPS molecules.

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?