Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Sign In to gain access to subscriptions and/or personal tools.
Journal of Endotoxin Research
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Kirikae, T.
Right arrow Articles by Nakano, M.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Kirikae, T.
Right arrow Articles by Nakano, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

LPS-dependent changes in the expression of 57 kDa and 53 kDa cell membrane proteins without participation of CD14

Teruo Kirikae

Department of Microbiology, Jichi Medical School, Tochigi-ken, Japan, tkirikae{at}ri.imcj.go.jp, Department of Infectious Diseases and Tropical Medicine, Research Institute, International Medical Centre of Japan, Shinjuku, Tokyo, Japan

Fumiko Kirikae

Department of Microbiology, Jichi Medical School, Tochigi-ken, Japan

Hiroki Iwai

Department of Microbiology, Jichi Medical School, Tochigi-ken, Japan

Nilofer Qureshi

Mycobacterial Research Laboratory, William S. Middleton Memorial Veterans Hospital, Madison, Wisconsin, USA

Koichi Fukase

Faculty of Science, Osaka University, Toyonaka, Japan

Shoichi Kusumoto

Faculty of Science, Osaka University, Toyonaka, Japan

Masayasu Nakano

Department of Microbiology, Jichi Medical School, Tochigi-ken, Japan

It is widely presumed that in addition to CD14, other molecules are necessary for lipopolysaccharide (LPS)-induced cell activation. In order to shed light on some of the biological and biochemical properties of these molecules, we examined the LPS responsiveness of CD14-negative, ST2 cells. Although ST2 cells do not express CD14 mRNA, they, nonetheless, expressed IL-6 mRNA and synthesized IL-6 protein when incubated with LPS in serum-free medium (i.e. without soluble CD14). Paxlitacel (TaxolTM) also induced IL-6 mRNA expression in ST2 cells, while Rhodobacter sphaeroides diphoshoryl lipid A (RsDPLA) inhibited both LPS- and Taxol-induced expression of IL-6 mRNA. Collectively, these data suggest that LPS, RsDPLA, and Taxol all recognize the same receptor complex on ST2 cells and do not require the participation of CD14. In addition, using antibody raised against the ST2 cell membrane fraction, we detected a set of LPS-specific membrane antigens in murine peritoneal macrophages, including two designated p57 (57 kDa) and p53 (53 kDa). There was no qualitative difference in the expression of p57 and p53 in LPS-responsive, C3H/HeN and LPS-hyporesponsive, C3H/HeJ macrophages. However, after stimulating the macrophages with LPS or Taxol, expression of p57 and p53 was diminished in C3H/HeN macrophages, but not in C3H/HeJ macrophages. Phorbol ester (PMA) and A23187 calcium ionophore did not suppress p57 or p53 expression, and the lipid A precursor, PE406, did not bind to either protein. Thus, p57 and p53 may play important roles in LPS-evoked responses, but they do not appear to serve as LPS receptors.

Journal of Endotoxin Research, Vol. 5, No. 1-2, 62-65 (1999)
DOI: 10.1177/09680519990050010301
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?