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Journal of Endotoxin Research
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Function of CD14 as a peptidoglycan receptor: differences and similarities with LPS

Roman Dziarski

Northwest Center for Medical Education, Indiana University School of Medicine, Gary, Indiana, USA

Dipika Gupta

Northwest Center for Medical Education, Indiana University School of Medicine, Gary, Indiana, USA

Peptidoglycan (PGN) is a macrophage activator from Gram-positive bacteria. PGN activates cells of hemopoietic origin through CD14 since: (i) PGN-unresponsive CD14-negative cells become PGNresponsive after transfection with CD14 and expression of membrane CD14; (ii) PGN binds to CD14 with high affinity; and (iii) anti-CD14 mAbs inhibit both binding of PGN to CD14 and activation of CD14-positive cells by PGN. However, there are several differences in the function of CD14 as PGN and LPS receptor: (i) the kinetics of binding are different; (ii) the affinity of binding in the absence of LPS-binding protein (LBP) is higher for PGN than LPS; (iii) LBP does not increase the affinity of binding of PGN to CD14 and does not enhance cell activation by PGN (in contrast to LPS); (iv) the regions of CD14 needed for binding and activation are partially similar and partially different for PGN and LPS; (v) sCD14:PGN complexes, in contrast to sCD14:LPS complexes, do not activate CD14-negative cells; (vi) PGN, in contrast to LPS, does not activate CHO cells expressing mCD14; and (vii) PGN and LPS induce differential activation of MAP kinases, but activate similar transcription factors (NF-{kappa}B, ATF1/CREB, and AP-1).

Journal of Endotoxin Research, Vol. 5, No. 1-2, 56-61 (1999)
DOI: 10.1177/09680519990050010201
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