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Detection of lipopolysaccharides electroblotted onto membranes

Division of Bacterial Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA

C.-M. Tsai

Division of Bacterial Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland, USA

Specific detection of LPS on nylon, as described in a previously published method (Chen W.H., Balakonis P., Tsai C-M. Detection of lipopolysaccharides blotted on nylon membranes. J Endotoxin Res 1995; 2: 405-410), has been performed with Amersham's Hybond-N. The current reformulated Hybond-N nylon membrane of Amersham did not work with the method. Magna nylon (Micron Separations Inc.) has been found as a successful substitute with results similar to that of the old Hybond-N. Incorporation of 0.05% SDS in the 25 mM Tris-192 mM glycine transfer buffer resulted in specific detection for LPS and proteins with a high content of carbohydrate. Such a phenomenon seems unique to nylon but not for PVDF and nitrocellulose.

Journal of Endotoxin Research, Vol. 4, No. 6, 459-462 (1997)
DOI: 10.1177/096805199700400610


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