This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Holst, O. Articles by Seltmann, G. Search for Related Content PubMed Articles by Holst, O. Articles by Seltmann, G. Social Bookmarking                         What's this?

Structural and serological characterisation of the O-specific polysaccharide of the lipopolysaccharide from proposed new serotype 029 of Serratia marcescens

Division of Medical and Biochemical Microbiology, Research Center Borstel, Center for Medicine and Biosciences, Borstel, Germany

H.M. Aucken

Laboratory of Hospital Infection, Public Health Laboratory Service, London, UK

G. Seltmann

Robert-Koch-Institut, Bundesinstitut für Infektionskrankheiten und nicht übertragbare Krankheiten, Bereich Wernigerode, Wernigerode, Germany

The structure of the repeating unit of the O-antigenic polysaccharide from the lipopolysaccharide (LPS) of Serratia marcescens strain 111 was determined by compositional and methylation analyses and NMR spectroscopy as 6)--D-Glcp-(12)--L-Rhap-(12)--L-Rha p-(12)--L-Rhap-(1 (Rha, rhamnose) which represents a new O-antigenic structure in LPS of S. marcescens but is similar to the structure of the repeating unit of the O-antigen of S. marcescens 018 6)--D-GlcpNAc-(12)--L-Rhap-(12)--L-Rha p-(12)--L-Rhap-(1 (Oxley D., Wilkinson S.G. Structure of a neutral polymer isolated from the lipopolysaccharide of the reference strain for S. marcescens serogroup 018. Carbohydr Res 1989; 195: 111-115). Serological investigations using S. marcescens O- and K-specific ELISA tests and immunoblotting showed that S111 reacted with anti-O18 and anti-K4 sera, and that the anti-S111 serum reacted with serotype strain 018. The K4 reaction was confirmed as capsular by the Quellung reaction. After absorption with the heterologous strain, S. marcescens 018 and strain 111 were shown to be immunologically distinct, probably because of the presence of the GlcNAc residue in 018. We propose to add S111 to the O-serotype strains of S. marcescens as 029.

Journal of Endotoxin Research, Vol. 4, No. 3, 215-220 (1997)
DOI: 10.1177/096805199700400307


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?