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Expression of acute phase proteins by bone marrow stromal cells

Department of Radiologic Technology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA, Department of Natural Sciences, Oklahoma Christian University, Oklahoma City, Oklahoma, USA

X. Wu

Immunobiology & Cancer Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA

M. Sullivan

Immunobiology & Cancer Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA

B.O. King

Department of Radiologic Technology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA

C.F. Webb

Immunobiology & Cancer Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA, Department of Microbiology & Immunology, University of Oklahma Health Sciences Center, Oklahoma City, Oklahoma, USA

J.M. Gimble

Immunobiology & Cancer Program, Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA, Department of Microbiology & Immunology, University of Oklahma Health Sciences Center, Oklahoma City, Oklahoma, USA, Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA, Department of Zoology, University of Oklahoma, Norman, Oklahoma, USA

The current work examines the expression of acute phase genes in a murine-derived bone marrow stromal cell model (BMS2) which exhibits adipocyte and osteoblast characteristics and supports lymphopoiesis in vitro. Each of these physiologic processes is responsive to inflammatory events such as endotoxemia. Exposure of BMS2 cells to pro-inflammatory cytokines induced the expression of the serum amyloid A and complement factor B. During adipocyte differentiation, expression of complement C3, complement factor D (adipsin), and angiotensinogen increased in a time dependent manner. The bone metabolic steroid, 1,25 dihydroxy vitamin D₃, specifically induced complement C3 expression in a time- and dose-dependent manner. Based on gel retention analysis, BMS2 nuclear extract contained proteins recognizing specific response elements from the complement C3, angiotensinogen, and complement factor B promoters. These results suggest that the bone marrow's repertoire of acute phase proteins is dependent on the stromal cell's phenotype or activation state.

Innate Immunity, Vol. 3, No. 5, 425-433 (1996)
DOI: 10.1177/096805199600300506


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