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Identification of N-3-hydroxypropionyl-2-O-methyl-D-perosamine as a specific constituent of the lipopolysaccharide from Vibrio bio-serogroup 1875 which has Ogawa antigen factor B of Vibrio cholerae O1

Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Saitama, Japan

Seiichi Kondo

Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Saitama, Japan

Yuji Haishima

Division of Microbiology, National Institute of Hygienic Sciences, Tokyo, Japan

Takehiro Iguchi

Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Saitama, Japan

Kazuhito Hisatsune

Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Saitama, Japan

A marine vibrio, designated Vibrio bio-serogroup 1875 Original (1875 Original), has both the Ogawa antigen factor B and the Inaba antigen factor C of Vibrio cholerae 01, in addition to its own O antigen factor. Its variant strain (1875 Variant) has the Inaba antigen factor C. The O polysaccharide chain (O-chain) of the lipopolysaccharide (LPS) isolated from this vibrio has been shown to consist of an (1 2)-linked homopolymer of N-3-hydroxypropionyl-D-perosamine. The present structural study revealed the presence of N-3-hydroxypropionyl-2- O-methyl-D-perosamine at the non-reducing terminus of the O-chain from the LPS of 1875 Original but not from that of the 1875 Variant. Since 2- O-methyl-D-perosamine is known as an Ogawa-specific constituent of the O-chain from V. cholerae O1 Ogawa O form, it appears that the presence of 2-O-methyl-D-perosamine at the non-reducing terminus of the (1 2)-linked D-perosamine homopolymer that forms the O-chain is responsible for the expression of the serological specificity of the Ogawa antigen factor B of 1875 Original.

Journal of Endotoxin Research, Vol. 3, No. 2, 143-149 (1996)
DOI: 10.1177/096805199600300207


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