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Journal of Endotoxin Research
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Specificity of monoclonal antibodies against Escherichia coli K-12 lipopolysaccharide

Lore Brade

Division of Biochemical Microbiology, Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, Borstel, Germany

Hans-Dieter Grimmecke

Division of Biochemical Microbiology, Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, Borstel, Germany

Otto Holst

Division of Biochemical Microbiology, Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, Borstel, Germany

Werner Brabetz

Division of Biochemical Microbiology, Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, Borstel, Germany

Alexander Zamojski

Institute of Organic Chemistry, Polish Academy of Sciences, Warsaw, Poland

Helmut Brade

Division of Biochemical Microbiology, Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, Borstel, Germany

Three monoclonal antibodies against the lipopolysaccharide (LPS) of Escherichia coli strain K-12 were obtained after immunization of BALB/c mice with heat-killed bacteria and were serologically characterized by hemagglutination, enzyme-immunoassay, Western, dot, and colony blot using as antigens bacteria, LPS, de-O-acylated LPS, dephosphorylated LPS, the core oligosaccharide and synthetic partial structures representing a di-, tri-and tetrasaccharide of the K-12 outer core region. In addition, deacylated LPS and synthetic oligosaccharides were covalently linked to bovine serum albumin resulting in artificial glycoconjugate antigens. In all assays used, the antibodies were specific for K-12 LPS. These antibodies detect E. coli K-12 LPS in its isolated form as well as on the surface of bacteria and are, thus, useful for all working with this microorganism.

Journal of Endotoxin Research, Vol. 3, No. 1, 39-47 (1996)
DOI: 10.1177/096805199600300105
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