Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Journal of Endotoxin Research
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Suda, Y.
Right arrow Articles by Kusumoto, S.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Suda, Y.
Right arrow Articles by Kusumoto, S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Separation of wild-type lipopolysaccharides by centrifugal partition chromatography: isolation of smooth form species

Yasuo Suda

Department of Chemistry, Faculty of Science, Osaka University, Osaka, Japan

Takaaki Shiyama

Department of Chemistry, Faculty of Science, Osaka University, Osaka, Japan

Takashi Yasukochi

Department of Chemistry, Faculty of Science, Osaka University, Osaka, Japan

Kazuhisa Kawano

Department of Chemistry, Faculty of Science, Osaka University, Osaka, Japan

Helmut Brade

Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, Borstel, Germany

Ernst Th. Rietschel

Forschungsinstitut Borstel, Institut für Experimentelle Biologie und Medizin, Borstel, Germany

Shoichi Kusumoto

Department of Chemistry, Faculty of Science, Osaka University, Osaka, Japan

The smooth (S)-form lipopolysaccharides (LPSs) having long O-polysaccharides were successfully isolated by centrifugal partition chromatography from the co-existing rough (R)-form species from the LPSs of wild-type strains of Salmonella enterica, serovars abortus equi and friedenau, and from Citrobacter freundii. Separation is based on different partition behaviors of the R- and S- form LPS species between aqueous and organic phases. The procedure described proved to be convenient, reproducible and readily applicable to large amounts of various LPS. It is another tool to study the relationship between chemical structure and biological activity of LPS.

Journal of Endotoxin Research, Vol. 3, No. 1, 1-7 (1996)
DOI: 10.1177/096805199600300101
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?