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Endotoxin tests in patients with sepsis

Viral Hepatitis and AIDS Laboratory, Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia, Department of Anaesthesiology, University of Malaya, Kuala Lumpur, Malaysia, Department of Paediatrics, University of Malaya, Kuala Lumpur, Malaysia, Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia, AGEN Biomedical Ltd, Acacia Ridge, Brisbane, Australia

M. Bhanumathy

Viral Hepatitis and AIDS Laboratory, Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia, Department of Anaesthesiology, University of Malaya, Kuala Lumpur, Malaysia, Department of Paediatrics, University of Malaya, Kuala Lumpur, Malaysia, Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia, AGEN Biomedical Ltd, Acacia Ridge, Brisbane, Australia

G.S.Y. Ong

Viral Hepatitis and AIDS Laboratory, Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia, Department of Anaesthesiology, University of Malaya, Kuala Lumpur, Malaysia, Department of Paediatrics, University of Malaya, Kuala Lumpur, Malaysia, Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia, AGEN Biomedical Ltd, Acacia Ridge, Brisbane, Australia

B.H. Yong

Viral Hepatitis and AIDS Laboratory, Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia, Department of Anaesthesiology, University of Malaya, Kuala Lumpur, Malaysia, Department of Paediatrics, University of Malaya, Kuala Lumpur, Malaysia, Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia, AGEN Biomedical Ltd, Acacia Ridge, Brisbane, Australia

N. Parasakthi

Viral Hepatitis and AIDS Laboratory, Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia, Department of Anaesthesiology, University of Malaya, Kuala Lumpur, Malaysia, Department of Paediatrics, University of Malaya, Kuala Lumpur, Malaysia, Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia, AGEN Biomedical Ltd, Acacia Ridge, Brisbane, Australia

M.T. Koh

Viral Hepatitis and AIDS Laboratory, Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia, Department of Anaesthesiology, University of Malaya, Kuala Lumpur, Malaysia, Department of Paediatrics, University of Malaya, Kuala Lumpur, Malaysia, Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia, AGEN Biomedical Ltd, Acacia Ridge, Brisbane, Australia

C.P. Siow

Viral Hepatitis and AIDS Laboratory, Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia, Department of Anaesthesiology, University of Malaya, Kuala Lumpur, Malaysia, Department of Paediatrics, University of Malaya, Kuala Lumpur, Malaysia, Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia, AGEN Biomedical Ltd, Acacia Ridge, Brisbane, Australia

J. Bosco

Viral Hepatitis and AIDS Laboratory, Department of Medical Microbiology, University of Malaya, Kuala Lumpur, Malaysia, Department of Anaesthesiology, University of Malaya, Kuala Lumpur, Malaysia, Department of Paediatrics, University of Malaya, Kuala Lumpur, Malaysia, Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia, AGEN Biomedical Ltd, Acacia Ridge, Brisbane, Australia

A new whole blood agglutination assay and a plasma Limulus amoebocyte lysate (LAL) assay for the measurement of endotoxin were evaluated for the diagnosis of sepsis and septic shock in 73 intensive care patients and 50 normal blood donors. All samples were blinded to the operators performing the tests. The endotoxin test results were compared with blood culture and clinical observations of sepsis progression. Endotoxin was detectable in 34 of 38 patients with culture positive Gram-negative bacteraemia, 2 of 11 patients with Gram-positive bacteraemia, 3 of the 7 patients with suspected sepsis, whose blood culture was repeatedly negative, and 1 of 17 non-septic patients. All blood donors were found to be negative for endotoxin. In 3 patients monitored during the course of their illness, the endotoxin assays correlated closely.

We conclude that endotoxin measurement was valuable in the care of patients with sepsis and that the whole blood agglutination assay was rapid and simple to perform.

Journal of Endotoxin Research, Vol. 2, No. 6, 387-393 (1995)
DOI: 10.1177/096805199600200601


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