This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Tufano, M.A. Articles by Rossano, F. Search for Related Content PubMed Articles by Tufano, M.A. Articles by Rossano, F. Social Bookmarking                         What's this?

Compartmentalization of intravesical and systemic interleukin-6 and tumor necrosis factor in mice stimulated with porins and lipopolysaccharide from Salmonella typhimurium

Istituto di Microbiologia, Facoltà di Medicina e Chirurgia, Seconda Università degli Studi di Napoli, and Istituto di Microbiologia, Università degli Studi di Messina. Italy

P. Catalanotti

Istituto di Microbiologia, Facoltà di Medicina e Chirurgia, Seconda Università degli Studi di Napoli, and Istituto di Microbiologia, Università degli Studi di Messina. Italy

C. Capasso

Istituto di Microbiologia, Facoltà di Medicina e Chirurgia, Seconda Università degli Studi di Napoli, and Istituto di Microbiologia, Università degli Studi di Messina. Italy

P. De Paolis

Istituto di Microbiologia, Facoltà di Medicina e Chirurgia, Seconda Università degli Studi di Napoli, and Istituto di Microbiologia, Università degli Studi di Messina. Italy

M. Ranieri

Istituto di Microbiologia, Facoltà di Medicina e Chirurgia, Seconda Università degli Studi di Napoli, and Istituto di Microbiologia, Università degli Studi di Messina. Italy

F. Rossano

Istituto di Microbiologia, Facoltà di Medicina e Chirurgia, Seconda Università degli Studi di Napoli, and Istituto di Microbiologia, Università degli Studi di Messina. Italy

Mucosal surfaces represent a natural colonization site for Gram-negative bacteria. We have already demonstrated the biologic role of Salmonella typhimurium porins in vitro and in vivo. In this paper we studied mucosal and systemic interleukin-6 (IL-6) and tumor necrosis factor (TNF) production in systematically challenged mice or after intravesical administration of LPS or porins from S. typhimurium. It was found that serum IL-6 levels increased in BALB/c mice 4 h after receiving either i.v. LPS or porins from S. typhimurium. The porin challenge was stronger. Serum IL-6 levels were higher after porins than after LPS. IL-6 was not detected in the urine of i.v. challenged mice. Findings regarding IL-6 urine levels in intravesically treated mice were comparable. In porin-challenged mice they decreased more slowly than the LPS-challenged ones. IL-6 was not detected in the serum of intravesically challenged mice. In i.v. LPS-challenged mice, serum TNF levels peaked earlier (at 2 h) than the IL-6 levels. A higher 2 h peak was instead seen in porin-challenged mice. TNF was not detected in the urine of i.v. challenged mice. With intravesical LPS challenge, urinary TNF levels peaked at 24 h, whereas in the porin-challenged mice the peak occurred 12 h earlier and was higher. Serum samples revealed no detectable TNF. These findings confirm that both porins and LPS activate the mucosal response, without any systemic involvement, as, for example, in patients with diseases such as pyelonephritis or gastroenteritis.

Journal of Endotoxin Research, Vol. 2, No. 5, 359-364 (1995)
DOI: 10.1177/096805199500200506


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?