Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Sign In to gain access to subscriptions and/or personal tools.
Journal of Endotoxin Research
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Neely, A.N.
Right arrow Articles by Holder, I.A.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Neely, A.N.
Right arrow Articles by Holder, I.A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

A murine model for studying endotoxemia and the efficacy of anti-LPS agents in an immunocompromised host

A.N. Neely

Shriners Burns Institute, Cincinnati, Ohio, USA

M.M. Orloff

Shriners Burns Institute, Cincinnati, Ohio, USA

A.R. Imwalle

Shriners Burns Institute, Cincinnati, Ohio, USA

I.A. Holder

Shriners Burns Institute, Cincinnati, Ohio, USA

In most murine models of endotoxemia, an exogenous agent is injected to increase the sensitivity of the mouse to endotoxin (lipopolysaccharide, LPS). Here, a clinically encountered event, a bum, was found to reproducibly decrease the amount of LPS required to kill half of the mice (LD50). In this more clinically relevant model, the anti-LPS agents, monophosphoryl lipid A and polymyxin B sulfate, each increased the LD50 of burned mice challenged with LPS from Klebsiella pneumoniae, while the LPS-directed monoclonal antibody E5 did not. However, E5 did protect burned mice challenged with smooth or rough LPS from Salmonella typhimurium and S. minnesota, respectively. Hence, in vivo protection was dependent upon both the anti-LPS agent and the chemical composition of the LPS used for intoxication. The differences in protection observed in this intoxication model may explain some protection discrepancies reported when these anti-LPS agents have been tested for protection against Gram negative sepsis.

Journal of Endotoxin Research, Vol. 2, No. 2, 115-120 (1995)
DOI: 10.1177/096805199500200206
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?