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Activation of Toll-like receptors 2 and 4 in Aspergillus fumigatus keratitisDepartment of Ophthalmology, Cardiovascular Remodeling and Function Research, Qilu Hospital, Shandong University, Jinan, China, Department of Ophthalmology, Shandong Provincial Hospital, Shandong University, Jinan, China
Department of Ophthalmology, Cardiovascular Remodeling and Function Research, Qilu Hospital, Shandong University, Jinan, China, xywu8868{at}163.com
The Kresge Eye Institute, Department of Ophthalmology, Wayne State University School of Medicine, Detroit, USA
Toll-like receptor (TLR) 2 and TLR4 are major receptors of Aspergillus fumigatus. Aspergillus fumigatus signaling in cornea induces the production of many pro-inflammatory molecules. In this study, we have shown that exposure of telomerase-immortalized human corneal epithelial cells (HCECs) to A. fumigatus antigens resulted in up-regulation of TLR2 and TLR4, and release of IL-1β and IL-10 in HCECs, effects that could be inhibited by treatment with TLR2, and TLR4 antibodies. In addition, the A. fumigatus antigens-induced production of IL-1β and IL-10 in supernatants of corneal epithelial cells was also attenuated by NF-
Key Words: Toll-like receptors • Aspergillus fumigatus • cytokines • NF-kappaB • keratitis
Innate Immunity, Vol. 15, No. 3,
155-168 (2009) |
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B inhibitor. Aspergillus fumigatus keratitis developed in Wistar rats, as evidenced by high SLE scores, influx of polymorphonuclear leukocytes (PMNs), activation of TLR2 and TLR4, and production of IL-1β and IL-10 over controls. These findings indicate that the cornea has functional TLR2 and TLR4, and activation of TLR2 and TLR4 through NF-