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Innate Immunity
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Peritoneal macrophages are important for the early containment of Enterococcus faecium peritonitis in mice

Masja Leendertse

Center for Infection and Immunity Amsterdam (CINIMA), Center for Experimental and Molecular Medicine, m.leendertse{at}amc.uva.nl, Department of Medical Microbiology

Rob J.L. Willems

Department of Medical Microbiology

Ida A.J. Giebelen

Center for Infection and Immunity Amsterdam (CINIMA), Center for Experimental and Molecular Medicine

Joris J.T.H. Roelofs

Department of Pathology, Academic Medical Center, Amsterdam, The Netherlands

Nico van Rooijen

Department of Molecular Cell Biology, Research Institute of Immunology & Inflammation, Faculty of Medicine, Free University, Amsterdam, The Netherlands

Marc J.M. Bonten

Department of Medical Microbiology, Julius Center for Health Studies and Primary Care, University Medical Center Utrecht, Utrecht, The Netherlands

Tom van der Poll

Center for Infection and Immunity Amsterdam (CINIMA), Center for Experimental and Molecular Medicine

The increasing incidence of infections with multi-drug resistant Enterococcus faecium necessitates studies to increase knowledge on the pathogenesis of these infections. In this study, the contribution of peritoneal macrophages during E. faecium peritonitis was investigated. In an ex vivo setting, peritoneal macrophages harvested from C57BL/6 mice were responsive to, and able to phagocytose and kill, E. faecium. In vivo, peritoneal macrophages were depleted by intraperitoneal injection of clodronate-encapsulated liposomes, prior to inducing E. faecium peritonitis. Depletion of resident peritoneal macrophages caused a clear delay in peritoneal clearance of E. faecium with increased systemic dissemination. Mice depleted of peritoneal macrophages were able to recruit macrophages and neutrophils to the peritoneal cavity after infection, comparable to control mice. Furthermore, increased levels of peritoneal cytokines and chemokines were found in mice depleted of peritoneal macrophages. This study indicates that peritoneal macrophages are important in the early containment of E. faecium peritonitis and for the regulation of the inflammatory response.

Key Words: Bacterial infection • Enterococcus faecium • macrophages • peritonitis

Innate Immunity, Vol. 15, No. 1, 3-12 (2009)
DOI: 10.1177/1753425908100238
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