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Hematological indices, inflammatory markers and neutrophil CD64 expression: comparative trends during experimental human endotoxemia

Department of Clinical Chemistry, Radboud University, Nijmegen Medical Centre, Nijmegen, The Netherlands, w.vandermeer{at}akc.umcn.nl

Peter Pickkers

Department of Intensive Care, Radboud University, Nijmegen Medical Centre, Nijmegen, The Netherlands

Colin S. Scott

Abbott Diagnostics Wiesbaden-Delkenheim

Johannes G. van der Hoeven

Department of Intensive Care, Radboud University, Nijmegen Medical Centre, Nijmegen, The Netherlands

Jacqueline Klein Gunnewiek

Department of Clinical Chemistry, Radboud University, Nijmegen Medical Centre, Nijmegen, The Netherlands

CD64 is a high-affinity FcRI receptor expressed by activated neutrophils that has been recently evaluated as a potential sepsis parameter. In the present study, the kinetics of neutrophil membrane CD64 expression were examined during a standardized inflammatory response, using a human endotoxemia model, and compared with hematological indices, CRP, cytokines and interleukins. Ten healthy subjects received 2 ng/kg intravenous Escherichia coli lipopolysaccharide (LPS). After administration of LPS, neutrophil CD64 showed a biphasic response, characterized by a first increase from 108.5 ± 7.5 to 133 ± 6 AFU after 1 h (P = 0.047) and a second increase that started at 6 h and reached its maximum of 167 ± 13 AFU at 22 h (P < 0.0001). CRP concentrations increased to 40 ± 5 mg/dl 22 h after the administration of LPS. The cytokines and interleukins reached their maximum response within 1—2 h. The maximum values of pro-inflammatory cytokines (TNF-, IFN- and IL-6) correlated with the CD64 expression at 22 h after LPS administration (r² = 0.76, r² = 0.78, r² = 0.81, respectively, all P < 0.05), whereas this correlation was not found for the anti-inflammatory IL-10 (r² = 0.058, P = 0.54), suggesting that neutrophil CD64 expression might be a quantitative marker for innate immunity that could easily be used in the clinical setting.

Key Words: CD64 • endotoxemia • neutrophils • sepsis markers

Journal of Endotoxin Research, Vol. 13, No. 2, 94-100 (2007)
DOI: 10.1177/0968051907079101


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