Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Sign In to gain access to subscriptions and/or personal tools.
Journal of Endotoxin Research
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Ulanova, M.
Right arrow Articles by Befus, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ulanova, M.
Right arrow Articles by Befus, D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Involvement of Syk protein tyrosine kinase in LPS-induced responses in macrophages

Marina Ulanova

Pulmonary Research Group, Department of Medicine, University of Alberta, Edmonton, Alberta, Canada, marina.ulanova{at}normed.ca

Samuel Asfaha

Pulmonary Research Group, Department of Medicine, University of Alberta, Edmonton, Alberta, Canada

Grant Stenton

Pulmonary Research Group, Department of Medicine, University of Alberta, Edmonton, Alberta, Canada

Alison Lint

Pulmonary Research Group, Department of Medicine, University of Alberta, Edmonton, Alberta, Canada

Deanna Gilbertson

Pulmonary Research Group, Department of Medicine, University of Alberta, Edmonton, Alberta, Canada

Alan Schreiber

University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA

Dean Befus

Pulmonary Research Group, Department of Medicine, University of Alberta, Edmonton, Alberta, Canada

Syk kinase is best known as a critical component of immunoreceptor signaling in leukocytes. Activation of Syk following cross-linking of Fc{gamma} and Fc{varepsilon} receptors on macrophages, mast cells, and other cells induces various inflammatory events. We hypothesized that Syk is involved in inflammatory responses induced by the lipopolysaccharide (LPS). We studied the role of Syk using its inhibition by antisense oligonucleotides, or small interfering RNA. Our data demonstrated that in vivo inhibition of Syk caused down-regulation of LPS-induced responses in rat alveolar macrophages. In in vitro experiments, inhibition of Syk in rat peritoneal macrophages, as well as in human myelomonocyte cell line THP-1 also caused a decrease in LPS-induced cytokine release. Our data support the hypothesis that, in macrophages, Syk is involved in LPS-induced intracellular signaling pathways leading to the release of pro-inflammatory mediators. Understanding the role of Syk in LPS-induced signaling may help in developing new therapeutic tools for inflammatory disorders.

Key Words: Cytokines • inflammation • lipopolysaccharide • macrophages • Syk protein tyrosine kinase

Journal of Endotoxin Research, Vol. 13, No. 2, 117-125 (2007)
DOI: 10.1177/0968051907079125
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?