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Journal of Endotoxin Research
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Stable isotope metabolic labeling of Neisseria meningitidis lipooligosaccharide

Deborah M.B. Post

The Buck Institute for Age Research, Novato, California, USA

DeSheng Zhang

The Inflammation Program

Jerrold P. Weiss

The Inflammation Program, Departments of Internal Medicine and Microbiology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, Iowa, USA, Veterans' Affairs Medical Center, Iowa City, Iowa, USA

Bradford W. Gibson

The Buck Institute for Age Research, Novato, California, USA, Department of Pharmaceutical Chemistry, University of California San Francisco, San Francisco, California, USA, bgibson{at}buckinstitute.org

The lipooligosaccharide (LOS) of a Neisseria meningitidis acetate auxotroph was metabolically labeled with either [2-13C]-sodium acetate or [1,2-13C 2]-sodium acetate. In this study, we demonstrated that this label was efficiently incorporated into both the lipid A acyl moieties and the two N-acetylglucosamines present in the oligosaccharide branch of the LOS. The development of this efficient labeling protocol should prove useful in future structural studies analyzing the interactions between LOS and host proteins.

Key Words: N. meningitidis • LOS • metabolic labeling

Journal of Endotoxin Research, Vol. 12, No. 2, 93-98 (2006)
DOI: 10.1177/09680519060120020501
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