Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Click here to sign up for SAGE Journal Email Alerts today!

Sign In to gain access to subscriptions and/or personal tools.
Journal of Endotoxin Research
This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Seya, T.
Right arrow Articles by Matsumoto, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Seya, T.
Right arrow Articles by Matsumoto, M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Antibodies against human Toll-like receptors (TLRs): TLR distribution and localization in human dendritic cells

Tsukasa Seya

Department of Microbiology and Immunology, Hokkaido University Graduate School of Medicine, Sapporo, Japan, seya-tu{at}med.hokudai.ac.jp, Department of Immunology, Osaka Medical Center for Cancer, Osaka, Japan

Kenji Funami

Department of Microbiology and Immunology, Hokkaido University Graduate School of Medicine, Sapporo, Japan

Mitsue Taniguchi

Department of Immunology, Osaka Medical Center for Cancer, Osaka, Japan

Misako Matsumoto

Department of Microbiology and Immunology, Hokkaido University Graduate School of Medicine, Sapporo, Japan, Department of Immunology, Osaka Medical Center for Cancer, Osaka, Japan

We have produced monoclonal antibodies (mAbs) against human Toll-like receptors (TLRs). These mAbs recognize the natural conformation of the extracellular domain leucine-rich repeats and, thereby, are suitable for immunoprecipitation, flow cytometric analysis and immunostaining. Using these mAbs, we determined the distribution of TLRs in a variety of human cell populations. Human TLRs that recognize bacterial components, particularly TLR-1, TLR-2, TLR-4 and TLR-6, reside on the cell surface and are expressed in myeloid and monocyte-derived dendritic cells (DCs) but not in plasmacytoid DCs (pDCs). Human TLR-3 resides in putative endosomes in myeloid DCs. Thus, the human myeloid DC subset harbors a unique and distinctive TLR repertoire. These mAbs will be useful to test the localization and distribution of human TLRs in a variety of cells and organs. Functional studies of human TLRs will also be feasible since certain of them are function-blocking mAbs.

Key Words: Monoclonal antibodies • human TLRs • TLR distribution • TLR localization • human dendritic cells

Journal of Endotoxin Research, Vol. 11, No. 6, 369-374 (2005)
DOI: 10.1177/09680519050110061301
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?