This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Alley, E.W. Articles by Russell, S.W. Search for Related Content PubMed Articles by Alley, E.W. Articles by Russell, S.W. Social Bookmarking                         What's this?

Contribution of clonally distinct subpopulations to heterogeneous production of inducible nitric oxide synthase by LPS-stimulated mouse macrophages

Wilkinson Laboratory for Cancer Research, University of Kansas Cancer Center, and the Departments of Microbiology, Molecular Genetics, and Immunology, and of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS, USA

X. Zhang

Wilkinson Laboratory for Cancer Research, University of Kansas Cancer Center, and the Departments of Microbiology, Molecular Genetics, and Immunology, and of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS, USA

S.W. Russell

Wilkinson Laboratory for Cancer Research, University of Kansas Cancer Center, and the Departments of Microbiology, Molecular Genetics, and Immunology, and of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS, USA

Expression of the inducible nitric oxide synthase (iNOS) gene in mouse macrophages can be induced by bacterial lipopolysaccharide (LPS). iNOS (EC 1.14.13.39) catalyzes production of the reactive nitrogen intermediate, nitric oxide (NO), which is very important to macrophage-mediated host defense in species such as the mouse and rat. We have investigated production of iNOS at the level of single cells through immunocytochemical analysis of LPS-stimulated macrophages. Both bone marrow culture-derived macrophages and those of the cell line, RAW 264.7, were examined. Heterogeneous production of iNOS within macrophage populations was explained in part by the existence of clones that were high producers of iNOS and, therefore, NO, while other clones were reproducibly low producers of each. All clonally derived populations continued to demonstrate heterogeneous iNOS production, suggesting that at least one additional mechanism must be responsible for the phenomenon of heterogeneity. In contrast to iNOS, LPS-stimulated macrophages synthesized interferonβ (IFNβ) uniformly throughout the population.

Journal of Endotoxin Research, Vol. 1, No. 4, 235-242 (1994)
DOI: 10.1177/096805199400100405


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				T. Ravasi, C. Wells, A. Forest, D. M. Underhill, B. J. Wainwright, A. Aderem, S. Grimmond, and D. A. Hume Generation of Diversity in the Innate Immune System: Macrophage Heterogeneity Arises from Gene-Autonomous Transcriptional Probability of Individual Inducible Genes J. Immunol., January 1, 2002; 168(1): 44 - 50. [Abstract] [Full Text] [PDF]

				J. J. Gao, M. B. Filla, M. J. Fultz, S. N. Vogel, S. W. Russell, and W. J. Murphy Autocrine/Paracrine IFN-{alpha}{beta} Mediates the Lipopolysaccharide-Induced Activation of Transcription Factor Stat1{alpha} in Mouse Macrophages: Pivotal Role of Stat1{alpha} in Induction of the Inducible Nitric Oxide Synthase Gene J. Immunol., November 1, 1998; 161(9): 4803 - 4810. [Abstract] [Full Text] [PDF]

				J. Gao, D. C. Morrison, T. J. Parmely, S. W. Russell, and W. J. Murphy An Interferon-gamma -activated Site (GAS) Is Necessary for Full Expression of the Mouse iNOS Gene in Response to Interferon-gamma and Lipopolysaccharide J. Biol. Chem., January 10, 1997; 272(2): 1226 - 1230. [Abstract] [Full Text] [PDF]