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Characterization and sequence analysis of the lsg (LOS synthesis genes) locus from Haemophilus influenzae type bDepartment of Microbiology, The University of Oklahoma Health Sciences Center, OK, USA, Department of Microbiology, University of Iowa, Iowa City, IA, USA, Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA, Department of Medicine, Division of Infectious Diseases, University of Indiana, Indianapolis, IN, USA
Department of Microbiology, The University of Oklahoma Health Sciences Center, OK, USA, Department of Microbiology, University of Iowa, Iowa City, IA, USA, Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA, Department of Medicine, Division of Infectious Diseases, University of Indiana, Indianapolis, IN, USA
Department of Microbiology, The University of Oklahoma Health Sciences Center, OK, USA, Department of Microbiology, University of Iowa, Iowa City, IA, USA, Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA, Department of Medicine, Division of Infectious Diseases, University of Indiana, Indianapolis, IN, USA
Department of Microbiology, The University of Oklahoma Health Sciences Center, OK, USA, Department of Microbiology, University of Iowa, Iowa City, IA, USA, Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA, Department of Medicine, Division of Infectious Diseases, University of Indiana, Indianapolis, IN, USA
Department of Microbiology, The University of Oklahoma Health Sciences Center, OK, USA, Department of Microbiology, University of Iowa, Iowa City, IA, USA, Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI, USA, Department of Medicine, Division of Infectious Diseases, University of Indiana, Indianapolis, IN, USA
Analysis of the lsg (LOS synthesis genes) cluster in Escherichia coli strain K12 and mutations in the lsg locus in Haemophilus influenzae type b indicated the presence of 3 regions responsible for sequential modifications of E. coli lipopolysaccharide (LPS). Sequencing of the lsg region yielded 7,435 bp that encompassed 7 complete and 1 partial open reading frames (ORFs 1-8). The predicted product of ORF1 had homology to the consensus sequence of cytochrome b proteins (21% identity, 51% similarity) and to other transmembrane proteins. The products of ORF5 and ORF6 share overall 23% identity and 49% similarity with each other. The ORF6 protein had high homology with the product of ORF275 of the E. coli rfb gene cluster (40% identity, 58% similarity), whose function is not known. Multiple sequence alignment of the ORF5 and ORF6 proteins with the RfbB, RfbJ and RfbX proteins revealed conserved motifs over the N-terminal half region of all these proteins. The products of ORF7 and ORF8 are homologous with Azotobacter vinelandii MolA protein (30% identity, 51% similarity) and MolB protein (26% identity, 48% similarity), respectively. The promoter regions of ORF1, 7 and 8 were determined by primer extension analysis and found to be similar to bacterial
Journal of Endotoxin Research, Vol. 1, No. 3,
165-174 (1994) This article has been cited by other articles:
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70-dependent promoters. ORF7 and ORF8 are transcribed into diverse orientation. At least 5 of the encoded proteins have been identified using coupled E. coli transcription/translation system and labeling with [35S]-methionine. We conclude that the genetic organization of the lsg biosynthesis pathway involves multiple operons that lead to the assembly of an H. influenzae LOS structure. 
