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Endotoxemia-related attenuation of interleukin-1 secretion in thermally injured patientsDepartment of Surgery, University of Toronto, Department of Immunology, and Sunnybrook Health Science Centre, University of Toronto, The Ross Tilley Burn Centre, The Wellesley Hospital, Toronto, Ontario, Canada
Department of Surgery, University of Toronto, Department of Immunology, and Sunnybrook Health Science Centre, University of Toronto, The Ross Tilley Burn Centre, The Wellesley Hospital, Toronto, Ontario, Canada
Department of Surgery, University of Toronto, Department of Immunology, and Sunnybrook Health Science Centre, University of Toronto, The Ross Tilley Burn Centre, The Wellesley Hospital, Toronto, Ontario, Canada
Department of Surgery, University of Toronto, Department of Immunology, and Sunnybrook Health Science Centre, University of Toronto, The Ross Tilley Burn Centre, The Wellesley Hospital, Toronto, Ontario, Canada
The present study examines the effect of post-burn endotoxemia on the capacity for the in vitro induction of interleukin-1 (IL-1)β synthesis by either inflammatory or non-inflammatory stimuli. Adherent cell (AC) cultures from patients with severe burns (n = 14, 25-65% total body surface area) were stimulated with LPS, phorbol ester (TPA) or IL-2 and studied simultaneously with intact or LPS-pretreated preparations from normal donors (n = 9). Levels of spontaneously released IL-1β in unstimulated AC cultures from patients and in LPS-pretreated preparations from normal individuals were highly elevated. However, LPS- and IL-2-related IL-1β production in such cultures was significantly decreased (P < 0.01-0.05). In contrast, in the majority of patients' cultures and LPS-pretreated normal AC preparations, TPA-induced IL-1β production remained relatively unaffected. In all patients studied, reduced capacity for the LPS-induced IL-1 production was observed concurrently with a significant (P < 0.05) elevation in plasma endotoxin content. Diminished secretion of IL-1β coincided also with a profound reduction of IL-2-dependent IFN
Journal of Endotoxin Research, Vol. 1, No. 2,
92-100 (1994) |
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production in patient cultures and in LPS-pretreated normal cultures. Thus, in the burn patient, endotoxemia-related specific desensitization may develop and, combined with other intrinsic mechanisms, restrict the pathophysiological sequelae of continuous bacterial stimulation.